Inheritance and Characterization of Rph27: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn.
Identifieur interne : 000058 ( Main/Exploration ); précédent : 000057; suivant : 000059Inheritance and Characterization of Rph27: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn.
Auteurs : Christopher T. Rothwell [Australie] ; Davinder Singh [Australie] ; Peter M. Dracatos [Australie] ; Robert F. Park [Australie]Source :
- Phytopathology [ 0031-949X ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
Abstract
The barley cultivar Quinn was previously reported to carry two genes for resistance to Puccinia hordei, viz. Rph2 and Rph5. In this study, we characterized and mapped a third resistance gene (RphCRQ3) in cultivar Quinn. Multipathotype testing in the greenhouse on a panel of barley genotypes previously postulated to carry Rph2 revealed rare race specificity in four genotypes in response to P. hordei pathotype (pt.) 222 P+ (virulent on Rph2 and Rph5). This suggested either the presence of a race-specific allele variant of Rph2 or the presence of an independent uncharacterized leaf rust resistance locus. A test of allelism on 1,271 F2 Peruvian (Rph2)/Quinn (Rph2 + Rph5) derived seedlings with P. hordei pt. 220 P+ (avirulent on Rph2 and virulent on Rph5) revealed no susceptible segregants. To determine whether the race-specific resistance in Quinn was due to an allele of Rph2 on chromosome 5H or a third uncharacterized resistance gene, we tested the Peruvian/Quinn F3 population with 222 P+ and observed monogenic inheritance. Subsequent bulked segregant analysis indicated the presence of complete in-phase marker fixation near the telomere on the short arm of chromosome 4H, confirming the presence of a third resistance locus in Quinn in addition to Rph2 and Rph5. In accordance with the rules and numbering system of barley gene nomenclature, RphCRQ3 has been designated Rph27.
DOI: 10.1094/PHYTO-12-19-0470-R
PubMed: 32096696
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inheritance and Characterization of <i>Rph27</i>: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn.</title><author><name sortKey="Rothwell, Christopher T" sort="Rothwell, Christopher T" uniqKey="Rothwell C" first="Christopher T" last="Rothwell">Christopher T. Rothwell</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Singh, Davinder" sort="Singh, Davinder" uniqKey="Singh D" first="Davinder" last="Singh">Davinder Singh</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Dracatos, Peter M" sort="Dracatos, Peter M" uniqKey="Dracatos P" first="Peter M" last="Dracatos">Peter M. Dracatos</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Park, Robert F" sort="Park, Robert F" uniqKey="Park R" first="Robert F" last="Park">Robert F. Park</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32096696</idno><idno type="pmid">32096696</idno><idno type="doi">10.1094/PHYTO-12-19-0470-R</idno><idno type="wicri:Area/Main/Corpus">000069</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000069</idno><idno type="wicri:Area/Main/Curation">000069</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000069</idno><idno type="wicri:Area/Main/Exploration">000069</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inheritance and Characterization of <i>Rph27</i>: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn.</title><author><name sortKey="Rothwell, Christopher T" sort="Rothwell, Christopher T" uniqKey="Rothwell C" first="Christopher T" last="Rothwell">Christopher T. Rothwell</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Singh, Davinder" sort="Singh, Davinder" uniqKey="Singh D" first="Davinder" last="Singh">Davinder Singh</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Dracatos, Peter M" sort="Dracatos, Peter M" uniqKey="Dracatos P" first="Peter M" last="Dracatos">Peter M. Dracatos</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Park, Robert F" sort="Park, Robert F" uniqKey="Park R" first="Robert F" last="Park">Robert F. Park</name><affiliation wicri:level="4"><nlm:affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW</wicri:regionArea><orgName type="university">Université de Sydney</orgName><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (MeSH)</term><term>Chromosome Mapping (MeSH)</term><term>Disease Resistance (MeSH)</term><term>Hordeum (MeSH)</term><term>Humans (MeSH)</term><term>Plant Diseases (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (MeSH)</term><term>Cartographie chromosomique (MeSH)</term><term>Hordeum (MeSH)</term><term>Humains (MeSH)</term><term>Maladies des plantes (MeSH)</term><term>Résistance à la maladie (MeSH)</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Basidiomycota</term><term>Chromosome Mapping</term><term>Disease Resistance</term><term>Hordeum</term><term>Humans</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Basidiomycota</term><term>Cartographie chromosomique</term><term>Hordeum</term><term>Humains</term><term>Maladies des plantes</term><term>Résistance à la maladie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The barley cultivar Quinn was previously reported to carry two genes for resistance to <i>Puccinia hordei</i>, viz. <i>Rph2</i> and <i>Rph5</i>. In this study, we characterized and mapped a third resistance gene (<i>RphCRQ3</i>) in cultivar Quinn. Multipathotype testing in the greenhouse on a panel of barley genotypes previously postulated to carry <i>Rph2</i> revealed rare race specificity in four genotypes in response to <i>P. hordei</i> pathotype (pt.) 222 P+ (virulent on <i>Rph2</i> and <i>Rph5</i>). This suggested either the presence of a race-specific allele variant of <i>Rph2</i> or the presence of an independent uncharacterized leaf rust resistance locus. A test of allelism on 1,271 F<sub>2</sub> Peruvian (<i>Rph2</i>)/Quinn (<i>Rph2</i> + <i>Rph5</i>) derived seedlings with <i>P. hordei</i> pt. 220 P+ (avirulent on <i>Rph2</i> and virulent on <i>Rph5</i>) revealed no susceptible segregants. To determine whether the race-specific resistance in Quinn was due to an allele of <i>Rph2</i> on chromosome 5H or a third uncharacterized resistance gene, we tested the Peruvian/Quinn F<sub>3</sub> population with 222 P+ and observed monogenic inheritance. Subsequent bulked segregant analysis indicated the presence of complete in-phase marker fixation near the telomere on the short arm of chromosome 4H, confirming the presence of a third resistance locus in Quinn in addition to <i>Rph2</i> and <i>Rph5</i>. In accordance with the rules and numbering system of barley gene nomenclature, <i>RphCRQ3</i> has been designated <i>Rph27</i>.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">32096696</PMID><DateCompleted><Year>2020</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>05</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>110</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>May</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Inheritance and Characterization of <i>Rph27</i>: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn.</ArticleTitle><Pagination><MedlinePgn>1067-1073</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-12-19-0470-R</ELocationID><Abstract><AbstractText>The barley cultivar Quinn was previously reported to carry two genes for resistance to <i>Puccinia hordei</i>, viz. <i>Rph2</i> and <i>Rph5</i>. In this study, we characterized and mapped a third resistance gene (<i>RphCRQ3</i>) in cultivar Quinn. Multipathotype testing in the greenhouse on a panel of barley genotypes previously postulated to carry <i>Rph2</i> revealed rare race specificity in four genotypes in response to <i>P. hordei</i> pathotype (pt.) 222 P+ (virulent on <i>Rph2</i> and <i>Rph5</i>). This suggested either the presence of a race-specific allele variant of <i>Rph2</i> or the presence of an independent uncharacterized leaf rust resistance locus. A test of allelism on 1,271 F<sub>2</sub> Peruvian (<i>Rph2</i>)/Quinn (<i>Rph2</i> + <i>Rph5</i>) derived seedlings with <i>P. hordei</i> pt. 220 P+ (avirulent on <i>Rph2</i> and virulent on <i>Rph5</i>) revealed no susceptible segregants. To determine whether the race-specific resistance in Quinn was due to an allele of <i>Rph2</i> on chromosome 5H or a third uncharacterized resistance gene, we tested the Peruvian/Quinn F<sub>3</sub> population with 222 P+ and observed monogenic inheritance. Subsequent bulked segregant analysis indicated the presence of complete in-phase marker fixation near the telomere on the short arm of chromosome 4H, confirming the presence of a third resistance locus in Quinn in addition to <i>Rph2</i> and <i>Rph5</i>. In accordance with the rules and numbering system of barley gene nomenclature, <i>RphCRQ3</i> has been designated <i>Rph27</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rothwell</LastName><ForeName>Christopher T</ForeName><Initials>CT</Initials><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Davinder</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dracatos</LastName><ForeName>Peter M</ForeName><Initials>PM</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-4199-7359</Identifier><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Robert F</ForeName><Initials>RF</Initials><AffiliationInfo><Affiliation>Plant Breeding Institute, School of Life and Environmental Science, University of Sydney, NSW, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="Y">Basidiomycota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001467" MajorTopicYN="Y">Hordeum</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">RphCRQ3</Keyword><Keyword MajorTopicYN="N">barley</Keyword><Keyword MajorTopicYN="N">bulked segregant analysis</Keyword><Keyword MajorTopicYN="N">genetics and resistance</Keyword><Keyword MajorTopicYN="N">leaf rust</Keyword><Keyword MajorTopicYN="N">race specificity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32096696</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-12-19-0470-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li></country><region><li>Nouvelle-Galles du Sud</li></region><settlement><li>Sydney</li></settlement><orgName><li>Université de Sydney</li></orgName></list><tree><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Rothwell, Christopher T" sort="Rothwell, Christopher T" uniqKey="Rothwell C" first="Christopher T" last="Rothwell">Christopher T. Rothwell</name></region><name sortKey="Dracatos, Peter M" sort="Dracatos, Peter M" uniqKey="Dracatos P" first="Peter M" last="Dracatos">Peter M. Dracatos</name><name sortKey="Park, Robert F" sort="Park, Robert F" uniqKey="Park R" first="Robert F" last="Park">Robert F. Park</name><name sortKey="Singh, Davinder" sort="Singh, Davinder" uniqKey="Singh D" first="Davinder" last="Singh">Davinder Singh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RustFungiV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000058 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000058 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RustFungiV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32096696
|texte= Inheritance and Characterization of Rph27: A Third Race-Specific Resistance Gene in the Barley Cultivar Quinn.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32096696" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RustFungiV1
| This area was generated with Dilib version V0.6.38. |  |